Filter-based assay for Escherichia coli in aqueous samples using bacteriophage-based amplification.
Identifieur interne : 000E46 ( Main/Exploration ); précédent : 000E45; suivant : 000E47Filter-based assay for Escherichia coli in aqueous samples using bacteriophage-based amplification.
Auteurs : Ratmir Derda [États-Unis] ; Matthew R. Lockett ; Sindy K Y. Tang ; Renee C. Fuller ; E Jane Maxwell ; Benjamin Breiten ; Christine A. Cuddemi ; Aysegul Ozdogan ; George M. WhitesidesSource :
- Analytical chemistry [ 1520-6882 ] ; 2013.
English descriptors
- KwdEn :
- Animals, Bacteriophages (physiology), Beverages (microbiology), Biosensing Techniques (methods), Citrus sinensis (chemistry), Color, Drinking Water (microbiology), Escherichia coli (isolation & purification), Escherichia coli (virology), Filtration (instrumentation), Filtration (methods), Limit of Detection, Milk (microbiology), Syringes, Water Microbiology.
- MESH :
- chemical , microbiology : Drinking Water.
- chemistry : Citrus sinensis.
- instrumentation : Filtration.
- isolation & purification : Escherichia coli.
- methods : Biosensing Techniques, Filtration.
- microbiology : Beverages, Milk.
- physiology : Bacteriophages.
- virology : Escherichia coli.
- Animals, Color, Limit of Detection, Syringes, Water Microbiology.
Abstract
This paper describes a method to detect the presence of bacteria in aqueous samples, based on the capture of bacteria on a syringe filter, and the infection of targeted bacterial species with a bacteriophage (phage). The use of phage as a reagent provides two opportunities for signal amplification: (i) the replication of phage inside a live bacterial host and (ii) the delivery and expression of the complementing gene that turns on enzymatic activity and produces a colored or fluorescent product. Here we demonstrate a phage-based amplification scheme with an M13KE phage that delivers a small peptide motif to an F(+), α-complementing strain of Escherichia coli K12, which expresses the ω-domain of β-galactosidase (β-gal). The result of this complementation-an active form of β-gal-was detected colorimetrically, and the high level of expression of the ω-domain of β-gal in the model K12 strains allowed us to detect, on average, five colony-forming units (CFUs) of this strain in 1 L of water with an overnight culture-based assay. We also detected 50 CFUs of the model K12 strain in 1 L of water (or 10 mL of orange juice, or 10 mL of skim milk) in less than 4 h with a solution-based assay with visual readout. The solution-based assay does not require specialized equipment or access to a laboratory, and is more rapid than existing tests that are suitable for use at the point of access. This method could potentially be extended to detect many different bacteria with bacteriophages that deliver genes encoding a full-length enzyme that is not natively expressed in the target bacteria.
DOI: 10.1021/ac400961b
PubMed: 23848541
Affiliations:
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Le document en format XML
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<front><div type="abstract" xml:lang="en">This paper describes a method to detect the presence of bacteria in aqueous samples, based on the capture of bacteria on a syringe filter, and the infection of targeted bacterial species with a bacteriophage (phage). The use of phage as a reagent provides two opportunities for signal amplification: (i) the replication of phage inside a live bacterial host and (ii) the delivery and expression of the complementing gene that turns on enzymatic activity and produces a colored or fluorescent product. Here we demonstrate a phage-based amplification scheme with an M13KE phage that delivers a small peptide motif to an F(+), α-complementing strain of Escherichia coli K12, which expresses the ω-domain of β-galactosidase (β-gal). The result of this complementation-an active form of β-gal-was detected colorimetrically, and the high level of expression of the ω-domain of β-gal in the model K12 strains allowed us to detect, on average, five colony-forming units (CFUs) of this strain in 1 L of water with an overnight culture-based assay. We also detected 50 CFUs of the model K12 strain in 1 L of water (or 10 mL of orange juice, or 10 mL of skim milk) in less than 4 h with a solution-based assay with visual readout. The solution-based assay does not require specialized equipment or access to a laboratory, and is more rapid than existing tests that are suitable for use at the point of access. This method could potentially be extended to detect many different bacteria with bacteriophages that deliver genes encoding a full-length enzyme that is not natively expressed in the target bacteria.</div>
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<tree><noCountry><name sortKey="Breiten, Benjamin" sort="Breiten, Benjamin" uniqKey="Breiten B" first="Benjamin" last="Breiten">Benjamin Breiten</name>
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<name sortKey="Fuller, Renee C" sort="Fuller, Renee C" uniqKey="Fuller R" first="Renee C" last="Fuller">Renee C. Fuller</name>
<name sortKey="Lockett, Matthew R" sort="Lockett, Matthew R" uniqKey="Lockett M" first="Matthew R" last="Lockett">Matthew R. Lockett</name>
<name sortKey="Maxwell, E Jane" sort="Maxwell, E Jane" uniqKey="Maxwell E" first="E Jane" last="Maxwell">E Jane Maxwell</name>
<name sortKey="Ozdogan, Aysegul" sort="Ozdogan, Aysegul" uniqKey="Ozdogan A" first="Aysegul" last="Ozdogan">Aysegul Ozdogan</name>
<name sortKey="Tang, Sindy K Y" sort="Tang, Sindy K Y" uniqKey="Tang S" first="Sindy K Y" last="Tang">Sindy K Y. Tang</name>
<name sortKey="Whitesides, George M" sort="Whitesides, George M" uniqKey="Whitesides G" first="George M" last="Whitesides">George M. Whitesides</name>
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<country name="États-Unis"><region name="Massachusetts"><name sortKey="Derda, Ratmir" sort="Derda, Ratmir" uniqKey="Derda R" first="Ratmir" last="Derda">Ratmir Derda</name>
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